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Image Search Results
Journal: Biological Research
Article Title: Betahistine alleviates benign paroxysmal positional vertigo (BPPV) through inducing production of multiple CTRP family members and activating the ERK1/2-AKT/PPARy pathway
doi: 10.1186/s40659-022-00385-3
Figure Lengend Snippet: Overexpression of CTRP1, 3, 6, 9 and 12 in mice with vestibular dysfunction vertigo. Mice of vestibular dysfunction vertigo were injected with pSB-T-CTRP1, 3, 6, 9, 12 or pSB-T empty vector, respectively. RT-qPCR was used to detect the mRNA expression of CTRP1 ( A ), CTRP3 ( B ), CTRP6 ( C ), CTRP9 ( D ) and CTRP12 ( E ). F and G Western blotting was used to evaluate the protein expression levels of CTRP1, 3, 6, 9 and 12. N = 4, Statistical differences were performed by using the Student’s t-test. * P < 0.05, ** P < 0.01
Article Snippet: CTRP-overexpression recombinant adenoviruses (up-Ad-CTRP, 2.0 × 10 10 pfu/mL) and
Techniques: Over Expression, Injection, Plasmid Preparation, Quantitative RT-PCR, Expressing, Western Blot
Journal: Biological Research
Article Title: Betahistine alleviates benign paroxysmal positional vertigo (BPPV) through inducing production of multiple CTRP family members and activating the ERK1/2-AKT/PPARy pathway
doi: 10.1186/s40659-022-00385-3
Figure Lengend Snippet: Overexpression of CTRP1, 3, 6, 9 and 12 reduces vestibular dysfunction in mice. The accuracy of air righting reflex and the scores of head tilt and swimming behavior were detected in mice treated with pSB-T-CTRP1 ( A and B ), pSB-T-CTRP3 ( C and D ), pSB-T-CTRP6 ( E and F ), pSB-T-CTRP9 ( G and H ) and pSB-T-CTRP12 ( I and J ), respectively. N = 3, Statistical differences were performed by using the Student’s t-test. * P < 0.05, ** P < 0.01
Article Snippet: CTRP-overexpression recombinant adenoviruses (up-Ad-CTRP, 2.0 × 10 10 pfu/mL) and
Techniques: Over Expression
Journal: Biological Research
Article Title: Betahistine alleviates benign paroxysmal positional vertigo (BPPV) through inducing production of multiple CTRP family members and activating the ERK1/2-AKT/PPARy pathway
doi: 10.1186/s40659-022-00385-3
Figure Lengend Snippet: Overexpression of CTRP1 and 12 promotes phosphorylation of ERK1/2 and AKT and expression of PPARγ. Mice of vestibular dysfunction vertigo were injected with pSB-T-CTRP1, 12 or pSB-T empty vector, respectively. A and B We detected the protein levels of p-ERK1/2 ( C ) and p-AKT ( D ) by using western blotting and evaluated the mRNA ( E ) and protein expression levels of PPARγ ( F ). N = 3, Statistical differences were performed by using the Student’s t-test. * P < 0.05, ** P < 0.01
Article Snippet: CTRP-overexpression recombinant adenoviruses (up-Ad-CTRP, 2.0 × 10 10 pfu/mL) and
Techniques: Over Expression, Phospho-proteomics, Expressing, Injection, Plasmid Preparation, Western Blot
Journal: Biochemical Genetics
Article Title: High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis
doi: 10.1007/s10528-022-10262-z
Figure Lengend Snippet: Effect of circ_0001821 silencing on cell biological properties was counteracted by overexpression of ISOC1. A Overexpression efficiency of ISOC1 was examined by western blot assay. B – J SW620 and HCT116 cells were transfected with si-NC, si-circ_0001821, si-circ_0001821 + pcDNA or si-circ_0001821 + ISOC1. B and C The proliferative capacity of the cells was measured by colony formation assay and EDU assay. D and E The cell migration and invasion capabilities were assessed by transwell assay. F Flow cytometry was used to detect apoptosis of cells. G and H Glucose consumption and lactate production in CRC cell lines were tested. I and J Western blot assay was used to assess the protein expression of PCNA, MMP2, Bax and HK2. ** p < 0.01, *** p < 0.001
Article Snippet: Isochorismatase domain containing 1 (ISOC1) overexpression vector (ISOC1) and
Techniques: Over Expression, Western Blot, Transfection, Colony Assay, EdU Assay, Migration, Transwell Assay, Flow Cytometry, Expressing